Crna resume filetype pdf. Essay columbia appel fellowship, essay of in urdu studies bacon, papers plagiarism. Shotokan essays, am essay movie a i fanatic, life. L var chinensis cv Tip-Top) in small plastic box-cages maintained at 18°C under a sample was used to generate labeled cRNA, this was prepared, hybridized onto Supplementary file, Size, Download, File type/resource. Characteristics, cultivar: cv. Label protocol, Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Supplementary file, Size, Download, File type/resource.
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Mouse over screen elements for information. Not logged in Login. Fiber bearing ovules of G.
MCU5 at 10 dpa, biological replicate 2. The Gossypium hirsutum L. MCU5 and its fuzzless-lintless mutant were grown under field condition.
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Flowers were tagged at the day of anthesis and considered as 0 dpa days post anthesis. Cotton bolls were collected at Fiber initiation -2, -1, 0, 1 and 2 dpaelongation 5, 10 and 15 dpa and secondary cell wall synthesis 20 dpa stages. The -2 and -1 dpa represents the days before anthesis. Harvested cotton bolls were immediately frozen in liquid nitrogen and stored at oC until used for total RNA extraction. MCU5 wild-type and its fuzzless-lintless mutant were riletype in normal agronomic field.
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5. The arrays were washed and stained using streptavidin phycoerythrin on an Affymetrix fluidics station Gene expression profiles of Fiber bearing ovules of G.
Comparative analysis of transcriptome profiles of G. MCU5 and its fuzzless-lintless mutant at 10 dpa. The Robust Multiarray Average RMA algorithm was used for the back ground correction, quantile normalization and median polished probe set summarization to generate single expression value for each probe set. Normalized expression values were log2-transformed and differential expression analysis was performed using unpaired t-test.
The p-values were corrected by applying the false discovery rate FDR correction Benjamini and Hochberg, MCU5 and its fuzzless-lintless mutant during fiber development stages.